Posts by Tag

TissueCyte

ChroMS Multicolor Imaging

less than 1 minute read

4 channel serial-section 2-photon and brainbow

Stopping the TissueCyte on laser modelock failure

less than 1 minute read

Code and instructions for stopping the TissueCyte upon laser modelock failure

Adjustable stage tilt on TissueCyte

3 minute read

How to add fine tip/tilt adjusters to the TissueCyte X/Y/Z stage

Changing the dichroics on the TissueCyte

1 minute read

How to change the dichroics on the TissueCyte

ScanImage on the TissueCyte

3 minute read

How to run ScanImage on the TissueCyte

Adding bandpass filters in front of the PMTs

2 minute read

How to add band pass filters on the TissueCyte

TissueCyte Beam Alignment: Primary Dichroic

1 minute read

How to align the excitation path dichroic on the TissueCyte

TissueCyte Beam Alignment: LCVR

1 minute read

Safely aligning the beam through the LCVR

Quick front/left routine for TissueCyte

1 minute read

Protocol for quickly finding the front/left position when imaging brains on a TissueCyte

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News

Multi-Scale Light-Sheet Fluorescence Microscopy for Fast Whole Brain Imaging

less than 1 minute read

Combined serial-sectioning plus lightsheet imaging

Multi-focal serial-section 2-photon imaging

less than 1 minute read

Super-fast serial section imaging by multiplexing

Allen Reference Atlas

less than 1 minute read

Creation of the Allen template brain and reference atlas

Reconstruction of 1000 projection neurons

less than 1 minute read

Janelia trace 1000 neurons and provide more details about their imaging system

Python-based image registration

less than 1 minute read

Fast Python-based image registration

On-line optical clearing method for whole-brain imaging in mice

less than 1 minute read

On-line clearing during serial section imaging

ChroMS Multicolor Imaging

less than 1 minute read

4 channel serial-section 2-photon and brainbow

FAST Spinning Disk Confical Protocol

less than 1 minute read

Detailed protocol for FAST serial section imaging

Imaging multiple samples at once

less than 1 minute read

BakingTray and StitchIt can now handle multiple samples at once

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Hacks

On-line optical clearing method for whole-brain imaging in mice

less than 1 minute read

On-line clearing during serial section imaging

ChroMS Multicolor Imaging

less than 1 minute read

4 channel serial-section 2-photon and brainbow

Imaging multiple samples at once

less than 1 minute read

BakingTray and StitchIt can now handle multiple samples at once

Stopping the TissueCyte on laser modelock failure

less than 1 minute read

Code and instructions for stopping the TissueCyte upon laser modelock failure

Adjustable stage tilt on TissueCyte

3 minute read

How to add fine tip/tilt adjusters to the TissueCyte X/Y/Z stage

Changing the dichroics on the TissueCyte

1 minute read

How to change the dichroics on the TissueCyte

ScanImage on the TissueCyte

3 minute read

How to run ScanImage on the TissueCyte

Adding bandpass filters in front of the PMTs

2 minute read

How to add band pass filters on the TissueCyte

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Sample prep

On-line optical clearing method for whole-brain imaging in mice

less than 1 minute read

On-line clearing during serial section imaging

ChroMS Multicolor Imaging

less than 1 minute read

4 channel serial-section 2-photon and brainbow

FAST Spinning Disk Confical Protocol

less than 1 minute read

Detailed protocol for FAST serial section imaging

Embedding without covalent agarose bonding

less than 1 minute read

How to cut thin sections without the covalent bonding protocol

Imaging multiple samples at once

less than 1 minute read

BakingTray and StitchIt can now handle multiple samples at once

Covalent Agarose Cross-Linking Protocol

less than 1 minute read

Our protocol for covalently cross-linking the brain to the agar

Embedding mold

less than 1 minute read

Easy agar embedding with aluminum mold

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Alignment

Stage alignment to imaging plane

2 minute read

How to ensure the imaging plane is aligned with the stage axes

Adjustable stage tilt on TissueCyte

3 minute read

How to add fine tip/tilt adjusters to the TissueCyte X/Y/Z stage

TissueCyte Beam Alignment: Primary Dichroic

1 minute read

How to align the excitation path dichroic on the TissueCyte

TissueCyte Beam Alignment: LCVR

1 minute read

Safely aligning the beam through the LCVR

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Maintenance

Stage alignment to imaging plane

2 minute read

How to ensure the imaging plane is aligned with the stage axes

TissueCyte Beam Alignment: Primary Dichroic

1 minute read

How to align the excitation path dichroic on the TissueCyte

TissueCyte Beam Alignment: LCVR

1 minute read

Safely aligning the beam through the LCVR

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Hints & Tips

Handy consumables

less than 1 minute read

List of useful consumables

Quick front/left routine for TissueCyte

1 minute read

Protocol for quickly finding the front/left position when imaging brains on a TissueCyte

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Registration

Python-based image registration

less than 1 minute read

Fast Python-based image registration

Registering widefield functional imaging data to the Allen Atlas

less than 1 minute read

Using blood vessel patterns to register in vivo data

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ScanImage

ScanImage on the TissueCyte

3 minute read

How to run ScanImage on the TissueCyte

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